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Srovnání antioxidačních vlastností a absorbce UV záření u extraktů vybraných bylin, Spiruliny a jejich směsí
Farinasso, Giulia
This study aimed to compare the antioxidant properties and UV radiation absorption of se-lected herbal extracts, blue-green algae spirulina extract and their mixtures. Extracts were ob-tained by ultrasound assisted extraction. The total polyphenol (TPC) and flavonoid contents (TFC) were determined, and the Trolox equivalent antioxidant capacity (TEAC) was meas-ured. The best antioxidant properties has been showed by C. officinalis individual extract and its mixture with spirulina. Additionally, the UV-absorbing substances at 355 nm were quanti-fied. The extracts from C. officinalis and S. nigra were further analysed for their rutin content by HPLC-DAD. The rutin content in S. nigra and C. officinalis extracts was found to be 1935,97 ± 98,39 μg.g-1 and 233,035 ± 8,839 μg.g-1 of dry weight of flos, respectively. The highest content of phenolic compounds was found in the S. nigra extract, and the highest total antioxidant capacity was determined for the C. officinalis extract. Pearson's correlation re-vealed that the overall antioxidant capacity and UV absorption results were significantly cor-related with the total phenolic compound content only for the elderflower extract. The syner-gistic effect of the herbs used in combination with spirulina was evaluated.
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Vliv železa a jeho bioakumulace v sinici Spirulina a mikrořase Chlorella
Koláčková, Kristýna
Iron is a trace element playing a key role in a number of metabolic pathways important for all living organisms. Iron deficiency is one of the most widespread problems in human nutrition. In order to possibly use Spirulina and Chlorella as dietary supplements for people suffering from iron deficiency sideropenic anemia, the effect of different concentrations of iron in the medium (0 mg/L, 5 mg/L and 10 mg/L) on its amount in the biomass was investigated. The optimal amount of Fe2+ in the medium was determined using spectrophotometric determination of optical density (OD) and iron content in Arthrospira maxima and Chlorella vulgaris biomass, which was determined by atomic absorption spectrometry (AAS). Expression analysis, performed in a model organism with a sequenced whole genome, Chlamydomonas reinhardtii, revealed the effect of selected concentrations of Fe2+ on the genes of the oxidative stress regulatory pathway. The results of the above analyzes show what are the optimal levels of iron cations for the growth of the studied organisms and its accumulation in them. The effect of iron in the chosen form, its assimilation and bioaccumulation in the cyanobacteria Spirulina and Chlorella microalgae was thus clarified.
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Možnosti průmyslového využití sinic a řas
Odehnalová, Jana
The bachelor thesis is elaborated in the form of literary research dealing with industrial use of algae and cyanobacteria. The first part of the thesis is devoted to the description and characteristics of individual algal species and cyanobacteria. The most important species used in the food industry are described here. The second part of the thesis deals with the processing of cyanobacteria and algae used to obtain important raw materials such as agar, carrageenan and alginates. There are also mentioned some types of these organisms used in the farm, in cosmetics production, in aquaculture, in wastewater treatment and as fertilizer and biofuel.
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